Renin inhibitors containing peptide isosteres

ABSTRACT

Renin inhibitory peptides of the formula ##STR1## and analogs thereof inhibit renin and are useful for treating various forms of renin-associated hypertension and hyperaldosteronism.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention is concerned with novel peptides which inhibit renin.

The present invention is also concerned with pharmaceutical compositions containing the novel peptides of the present invention as active ingredients, with methods of treating renin-associated hypertension and hyperaldosteronism, with diagnostic methods which utilize the novel peptides of the present invention, and with methods of preparing the novel peptides of the present invention.

Renin is a proteolytic enzyme of molecular weight about 40,000, produced and secreted by the kidney. It is secreted by the juxtaglomerular cells and acts on the plasma substrate, angiotensinogen, to split off the decapeptide angiotensin I, which is converted to the potent pressor agent angiotensin II. Thus, the renin-angiotensin system plays an important role in normal cardiovascular homeostasis and in some forms of hypertension.

In the past, attempts to modulate or manipulate the renin-angiotensin system have met with success in the use of inhibitors of angiotensin I converting enzyme. In view of this success, it seems reasonable to conclude that a specific inhibitor of the limiting enzymatic step that ultimately regulates angiotensin II production, the action of renin on its substrate, would be at least equally successful. Thus, an effective inhibitor of renin has been long sought as both a therapeutic agent and as an investigative tool.

2. Brief Description of the Prior Art

There has been substantial interest in the synthesis of useful renin inhibitors for many decades; and the following table lists the major classes of renin inhibitors that have been studied, as well as their inhibition constants (K_(i)):

    ______________________________________                                         Class                K.sub.i (M)                                               ______________________________________                                         Renin antibody       probably 10.sup.-6                                        Pepstatin            10.sup.-6 -10.sup.-7                                      Phospholipids        10.sup.-3                                                 Substrate analogs                                                              Tetrapeptides        10.sup.-3                                                 Octa- to tridecapeptides                                                                            10.sup.-5 -10.sup.-6                                      ______________________________________                                    

Umezawa et al., in J. Antibiot. (Tokyo) 23: 259-262, 1970, reported the isolation of a peptide from actinomyces that was an inhibitor of aspartyl proteases such as pepsin, cathepsin D, and renin. This peptide, known as pepstatin, was found by Gross et al., Science 175: 656, 1971, to reduce blood pressure in vivo after the injection of hog renin into nephrectomized rats. However, pepstatin has not found wide application as an experimental agent because of its limited solubility and its inhibition of a variety of other acid proteases in addition to renin. The structure of pepstatin is shown below: ##STR2##

To date, many efforts have been made to prepare a specific renin inhibitor based on substrate analogy. Since the human renin substrate has only recently been elucidated (Tewksbury et al., Circulation 59, 60, Supp. II: 132, October 1979), heretofore substrate analogy has been based on the known pig renin substrate. While the human and pig renin substrates are not the same, the substrate analogy based on pig renin has always been considered acceptable in the art as predictive of human renin inhibitory activity because of the closely related activity of the two renins. Thus, while pig renin does not cleave the human renin substrate, human renin, on the other hand, does cleave the pig renin substrate. See Poulsen et al., Biochim. Biophys. Acta 452: 533-537, 1976; and Skeggs, Jr. et al., J. Exp. Med. 106: 439-453, 1957. Moreover, the human renin inhibitory activity of the peptides of the present invention most active in inhibiting pig renin has been confirmed, thus providing further evidence of this accepted correlation between human and pig renin activity.

It has been found, for example, using pig renin substrate analogy, that the octapeptide sequence extending from histidine-6 through tyrosine-13 has kinetic parameters essentially the same as those of the full tetradecapeptide renin substrate. The amino acid sequence of the octapeptide in pig renin substrate is as follows: ##STR3## Renin cleaves this substrate between Leu¹⁰ and Leu¹¹.

Kokubu et al., Biochem. Pharmacol. 22: 3217-3223, 1973, synthesized a number of analogs of the tetrapeptide found between residues 10 to 13, but while inhibition could be shown, inhibitory constants were only of the order of 10⁻³ M.

Analogs of a larger segment of renin substrate were also synthesized: Burton et al., Biochemistry 14: 3892-3898, 1975, and Poulsen et al., Biochemistry 12: 3877-3882, 1973. Two of the major obstacles which had to be overcome to obtain an effective renin inhibitor useful in vivo were lack of solubility and weak binding (large inhibitory constant). Modifications to increase solubility soon established that the inhibitory properties of the peptides are markedly dependent on the hydrophobicity of various amino acid residues, and that increasing solubility by replacing lipophilic amino acids with hydrophilic isosteric residues becomes counterproductive. Other approaches to increasing solubility have had limited success. Various modifications designed to increase binding to renin have also been made, but here too, with only limited success. For a more detailed description of past efforts to prepare an effective inhibitor of renin, see Haber and Burton, Fed. Proc. Fed. Am. Soc. Exp. Biol. 38: 2768-2773, 1979.

More recently, Szelke et al., in work described in European Patent Publication No. 45,665; Nature, 299, 555 (1982); Hypertension, 4, Supp. 2, 59, 1981; British Pat. No. 1,587,809; and "Novel Transition-State Analogue Inhibitors of Renin", a presentation at the Eighth American Peptide Symposium, May 22-27, 1983, Tucson, Ariz., have replaced the Leu-Leu site of renin cleavage by isosteric substitution, and obtained compounds with excellent potency.

Powers et al., in Acid Proteases, Structure, Function and Biology, Plenum Press, 1977, 141-157 have suggested that in pepstatin, statine occupies the space of the two amino acids on either side of the cleavage site of a pepsin substrate, and Tang et al., in Trends in Biochem. Sci., 1: 205-208 (1976) and J. Biol. Chem., 251: 7088-94, 1976, have proposed that the statine residue of pepstatin resembles the transition state for pepsin hydrolysis of peptide bonds. However, the applicability of these concepts of renin inhibitors is not taught in any of these refernces, and would be speculative due to the known high degree of specificity of the renin enzyme.

Preparation and use of peptide isosteres of the type employed in the present invention is described in the following references, which do not, however, disclose or suggest the renin inhibitors of the present invention: Natarajan et al., Peptides. Synthesis-Structure-Function, ed. D. H. Rich and E. Gross, Pierce Chem. Co., Rockford, Ill., pp. 429-433, 1981; Ondetti and Cushman, Biopolymers, 20: 2001-2010, 1981; Ondetti et al., Chemistry and Biology of Peptides, ed. J. Meienhofer, Ann Arbor Science, pp. 525-531, 1972; Van Lommen et al., European Peptide Symposium 16th, Peptides 1980, ed. K. Brunfeldt, Scriptor, Copenhagen, pp. 248-252, 1981; Almquist et al., J. Med. Chem., 23: 1392-1398, 1980; Kawasaki and Maeda, Biochem. Biophys. Res. Commun. 106: 113-116, 1982; Fok and Yankellov, Biochem. Biophys. Res. Commun. 74: 273-278, 1977; Spatola et al., Peptides. Structure-Function-Biological Function, eg. E. Gross and J. Meienhofer, Pierce Chem. Co., Rodkford, Ill., pp. 273-276, 1979; Spatola and Bettag. J. Org. Chem. 46: 2393-2394, 1981; Hann et al., J. Chem. Soc. Chem. Commun., 234-235, 1980; Cox et al., J. Chem. Soc. Chem. Commun., 799-800, 1980; Atherton et al., J. Chem. Soc. (C), 3393-3396, 1971; Parry et al., Chemistry and Biology of Peptides, ed. J. Meienhofer, Ann Arbor Science, pp. 541-544, 1972; Hudson et al., Int. J. Peptide Protein Res. 15: 122-129, 1979; Frank and Desiderio, Anal. Biochem. 90: 413-419, 1978; Jacobson and Bartlett, JACS, 103: 654-657, 1981; Jennings-White and Almquist, Tet. Lett. 23: 2533-2534, 1982; Morton et al., Tet. Lett., 23: 4123-4126, 1982; Rich et al., Biochem. Biophys. Res. Comm. 104: 1127-1133, 1982; and Spatola et al., Proceedings of the Seventh American Peptide Symposium, ed. R. H. Rich and E. Gross, pp. 613-616, 1981.

Veber and Rich, in U.S. Pat. No. 4,384,994 and published European Patent Application No. 0,077,029; Evans and Rittle, in U.S. Pat. No. 4,397,786; Veber and Boger, in publsihed European Patent Application No. 0,077,028; Boger et al, Nature, 303: 81-84 (1983); have all described renin inhibitory peptides containing statine. However, none of these references describe or suggest the renin inhibitors containing peptide isosteres of the present invention and the significant increase in renin inhibitory activity obtainable therewith.

For other articles describing previous efforts to devise renin inhibitors, see Marshall, Federation Proc. 35: 2494-2501, 1976; Burton et al., Proc. Natl. Acad. Sci. USA 77: 5476-5479, September 1980; Suketa et al., Biochemistry 14: 3188, 1975; Swales, Pharmac. Ther. 7: 173-201, 1979; Kokubu et al., Nature 217: 456-457, Feb. 3, 1986; Matsushita et al., J. Antibiotics 28: 1016-1018, December 1975; Lazar et al., Biochem. Pharma. 23: 2776-2778, 1974; Miller et al., Biochem. Pharma. 21: 2941-2944, 1972; Haber, Clinical Science 59: 7s-19s, 1980; Rich et al., J. Org. Chem. 43: 3624, 1978, and J. Med. Chem. 23: 27, 1980; Burton et al., U.S. Pat. No. 4,269,827; Castro et al., U.S. Pat. No. 4,185,096; and Sankyo Jap. Pat. No. 76-067001.

DETAILED DESCRIPTION OF THE INVENTION AND PREFERRED EMBODIMENTS

In accordance with the present invention there are provided renin inhibitory peptides of the formula: ##STR4## wherein: A is hydrogen; or ##STR5## where X is ##STR6## and R_(a) ² and R_(b) ² may be the same or different and are hydrogen; W--(CH₂)_(n) -- or W--(CH₂)_(m) --CH═CH--(CH₂)_(p) --(CH₂)_(p), where W is hydrogen; C₁₋₄ alkyl; aryl;

C₃₋₇ cycloalkyl; or C₃₋₇ cycloalkyl or aryl substituted with up to five members independently selected from the group consisting of C₁₋₈ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; n is 0 to 5; m is 0 to 2; and p is 0 to 2; except that where X is --O--, only one of R_(a) ² or R_(b) ² is present;

B is absent; glycyl; sarcosyl; or ##STR7## where R¹ is as defined further below; D is absent; or ##STR8## where Z is --(CH₂)₁ -- and l is 1 or 2; or --S--; E is absent; or ##STR9## where m is 1 to 4; and R⁵ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl where the aryl portion is substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl;

G is ##STR10## where q is 1 to 4; R⁴ is as defined further below;

R⁶ is C₃₋₆ alkyl; C₃₋₇ cycloalkyl; aryl; or C₃₋₇ cycloalkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; and

Q is ##STR11## wherein X' is hydroxy; amino; or mono- or di-C₁₋₄ alkyl amino; and W' is absent; --O--; --NH--; or --CH₂ --; ##STR12## where X" and X"' are independently absent; or ##STR13## and W" is absent; --CH₂ --; or ##STR14## where R⁸ is hydrogen or C₁₋₃ alkyl; ##STR15## where R is hydrogen; C₁₋₄ alkyl; formyl; C₁₋₄ alkanoyl; aroyl; carboxy; C₁₋₄ alkoxycarbonyl; aryloxycarbonyl; or aryl C₁₋₄ alkoxycarbonyl;

J is (1) --Y--(CH₂)_(n) --R⁷

where

Y is --NH-- or --O--;

n is 0 to 5; and

R⁷ is hydrogen; hydroxy; C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- or di-C₁₋₄ alkylamino, and halo; amino; mono-, di-, or tri-C₁₋₄ alkylamino; guanidyl; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino;

(2) ##STR16## where Y is as defined above;

n is 0 or 1;

n' is 0 or 1;

n" is 1 to 4;

n"' is 1 to 4; and

R⁷ and R_(a) ⁷ may be the same or different and have the same meaning as R⁷ above and R_(a) ⁷ may additionally be ##STR17## where R⁸ is hydrogen or C₁₋₃ alkyl; (3) ##STR18## where Y is as defined above;

n is 0 or 1; and

Z' is ##STR19## where n is 0 or 1; and

R⁸ is as defined above; or ##STR20## where n is 0 or 1;

(4) ##STR21## where R⁵ is as defined above; and R⁹ is hydrogen; C₁₋₄ alkyl; hydroxy; or C₃₋₇ cycloalkyl; or

(5) ##STR22## where Y is --NH-- or --O--;

q is 1-5;

q' is 0-5;

R¹⁰ is hydrogen; hydroxy; N(R')₂, where

R' may be the same or different and is hydrogen or C₁₋₄ alkyl; guanidyl; or N.sup.⊕ (R')₃ A.sup.⊖, where R' is as defined above, and A.sup.⊖ is a counterion; provided that at least one R¹⁰ is not hydrogen;

R¹¹ is C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to three members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- di-C₁₋₄ alkylamino, amino C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; carboxy, ester or amide; sulfo; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino;

R¹² is hydrogen; or carboxy, ester or amide;

R¹³ is carboxy, ester or amide; sulfo; or aryl substituted with up to three members selected from the group consisting of amino-C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ -alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; and

R¹⁴ is carboxy, ester or amide; or ##STR23## where Y is --NH-- or --O--;

k is 0-4;

k' is 0 or 1;

k" is 0-4;

k"' is 0 or 1;

R' is hydrogen or C₁₋₄ alkyl; and

R" is hydrogen or C₁₋₄ alkyl;

L is absent; OR"; NHR"; or N(R")₂, where R" may be the same or different and is hydrogen or C₁₋₄ alkyl; provided that, B and/or L are/is present only when J is defined as in (4) thereunder;

R¹ is hydrogen; C₁₋₄ alkyl; hydroxy C₁₋₄ alkyl; aryl; aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; indolyl; 4-imidazolyl; amino C₂₋₄ alkyl; acyl C₂₋₄ alkyl wherein the acyl is ##STR24## and R⁹ is as defined above; guanidyl C₂₋₃ alkyl; or methylthiomethyl;

R⁴ is hydrogen; or ##STR25## where R⁹ is as defined above; and R³ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl; and

wherein all of the asymmetric carbon atoms have an S configuration, except for those in the B, D, and Q portion of G substituents, which may have an S or R configuration; and a pharmaceutically acceptable salt thereof.

While both the S and R chiralities for asymmetric carbon atoms in the B substituent are included in the peptides of the present invention, preferred chiralities are indicated in the description which follows.

In the above definitions, the term "alkyl" is intended to include both branched and straight chain hydrocarbon groups having the indicated number of carbon atoms.

The term "halo" means fluoro, chloro, bromo and iodo.

The aryl substituent represents phenyl, and naphthyl.

The heterocyclic substituent recited above represents any 5- or 6-membered ring containing from one to three heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur; having various degrees of unsaturation; wherein the nitrogen and sulfur heteroatoms may optionally be oxidized; wherein the nitrogen heteroatom may optionally be quaternized; and including any bicyclic group in which any of the above heterocyclic rings is fused to a benzene ring. Heterocyclic substituents in which nitrogen is the heteroatom are preferred, and of these, those containing a single nitrogen atom are preferred. Fully saturated heterocyclic substituents are also preferred. Thus, piperidine is a preferred heterocyclic substituent. Other preferred heterocyclic substituents are: pyrryl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, piperidinyl, pyrazinyl, piperazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, furyl, thienyl and benzothienyl.

Where the heterocyclic substituent itself is substituted, it is preferred that the substituent be aryl C₁₋₄ alkyl.

The novel renin inhibitory peptides of the present invention may also be described in terms of common amino acid components and closely related analogs thereof, in accordance with the following formula:

    A-B-B-D-E-Y-G-Z-J-B-L                                      (II.)

The A, B, D, G, J and L components correspond to the same portions of Formula I.

The common amino acid components of Formula II are as follows:

A has the same meaning as above in Formula I;

B is Ala, Leu, Ser, Thr, Phe, Tyr, Trp, His, Lys, Orn, Arg, or Met;

D is Pro;

E is Ala, Leu, Phe, HomoPhe, BisHomoPhe, Tyr, HomoTyr, Trp, or HomoTrp;

Y is the same as B;

G has the same meaning as above in Formula I;

Z is Ala, Leu, Phe, Tyr, Trp, Ser, Gly, Val, Ile, or Thr;

J has the same meaning as above in Formula I; and

L has the same meaning as above in Formula I.

It will be understood that closely related analogs of the above common amino acids, for example, aliphatic amino acids in addition to Ala, Val, Leu, and Ile, such as α-aminobutyric acid (Abu), and substituted phenyl derivatives of Phe, are included in the broad description of the novel inhibitory peptides of the present invention represented by Formula I and its definitions. Thus, the peptides of Formula II and its definitions represent preferred peptides of the present invention.

Preferred inhibitory peptides of the present invention are the following: ##STR26##

The inhibitory peptides of the present invention may be better appreciated in terms of substrate analogy from the following illustration of Formula I alongside the octapeptide sequence of a portion of the pig renin substrate, which renin cleaves between Leu¹⁰ and Leu¹¹ : ##STR27##

As can be seen, a unique aspect and essential feature of the present invention is the substitution of a peptide isostere component for the double amino acid sequence: Leu¹⁰ -Leu¹¹ in the endogenous pig renin substrate. It is believed that substitution of this isostere component for both leucine amino acids rather than just one leucine results in an improved substrate analogy due to the greater linear extent of the isostere component as compared to a single leucine component. Thus, the isostere component more closely approximates Leu-Leu in linear extent, and thereby provides a better "fit" to the renin enzyme.

The inhibitory peptides of the present invention may also be better appreciated in terms of substrate analogy from the following illustration of Formula I alongside the octapeptide sequence of a portion of the human renin substrate, which renin cleaves between Leu¹⁰ and Val¹¹ : ##STR28##

As can be seen, a unique aspect and essential feature of the present invention is the substitution of a peptide isostere component for the double amino acid sequence: Leu¹⁰ -Val¹¹ in the endogenous human renin substrate. It is believed that substitution of this isostere component for both the leucine and valine amino acids rather than just the leucine results in an improved substrate analogy due to the greater linear extent of the isostere component as compared to a single leucine component. Thus, the isostere component more closely approximates Leu-Val in linear extent, and thereby provides a better "fit" to the human renin enzyme.

In the endogenous substrate it is also preferred to substitute Leu for Val¹² and Phe for Tyr¹³ in order to enhance the inhibitory activity of the resulting peptide.

The Formula I compounds can be used in the form of salts derived from inorganic or organic acids and bases. Included among such acid addition salts are the following: acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, tosylate, and undecanoate. Base salts include ammonium salts, alkali metal salts such as sodium and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases such as dicyclohexylamine salts. N-methyl-D-glucamine, and salts with amino acids such as arginine, lysine, and so forth. Also, the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chloride, bromides and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl; and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides like benzyl and phenethyl bromides and others. Water or oil-soluble or dispersible products are thereby obtained.

The present invention is also directed to combinations of the novel renin-inhibitory peptides of Formula I with one or more antihypertensive agents selected from the group consisting of diuretics, α and/or β-adrenergic blocking agents. CNS-acting agents, adrenergic neuron blocking agents, vasodilators, angiotensin I converting enzyme inhibitors, and other antihypertensive agents.

For example, the compounds of this invention can be given in combination with such compounds or salt or other derivative forms thereof as:

Diuretics: acetazolamide; amiloride; bendroflumethiazide; benzthiazide; bumetanide; chlorothiazide; chlorthalidone; cyclothiazide; ethacrynic acid; furosemide; hydrochlorothiazide; hydroflumethiazide; indacrinone (racemic mixture, or as either the (+) or (-) enantiomer alone, or a manipulated ratio, e.g., 9:1 of said enantiomers, respectively); metolazone; methyclothiazide; muzolimine; polythiazide; quinethazone; sodium ethacrynate; sodium nitroprusside; spironolactone; ticrynafen; triamterene; trichlormethiazide;

α-Adrenergic Blocking Agents: dibenamine; phentolamine; phenoxybenzamine; prazosin; tolazoline;

β-Adrenergic Blocking Agents: atenolol; metoprolol; nadolol; propranolol; timolol;

((±)-2-[3-(tert-butylamino)-2-hydroxypropoxy]-2-furananilide) (ancarolol);

(2-acetyl-7-(2-hydroxy-3-isopropylaminopropoxy)benzofuran HCL) (befunolol);

((±)-1-(isopropylamino)-3-(p-(2-cyclopropylmethoxyethyl)-phenoxy)-2-propranol HCl) (betaxolol);

(1-[(3,4-dimethoxyphenethyl)amino]-3-(m-tolyloxy)-2-propanol HCl) (bevantolol);

(((±)-1-(4-((2-isopropoxyethoxy)methyl)phenoxy)-3-isopropylamino-2-propanol)fumarate) (bisoprolol);

(4-(2-hydroxy-3-[4-(phenoxymethyl)-piperidino]propoxy)-indole);

(carbazolyl-4-oxy-5,2-(2-methoxyphenoxy)-ethylamino-2-propanol);

(1-((1,1-dimethylethyl)amino)-3-((2-methyl-1H-indol-4-yl)oxy)-2-propanol benzoate) (bopindolol);

(1-(2-exobicyclo[2.2.1]-hept-2-ylphenoxy)-3-[(1-methylethyl)-amino]-2-propanol HCl) (bornaprolol);

(o-[2-hydroxy-3-[(2-indol-3-yl-1,1-dimethylethyl)amino]propoxy]benzonitrile HCl) (bucindolol);

(α-[(tert.butylamino)methyl]-7-ethyl-2-benzofuranmethanol) (bufuralol);

(3-[3-acetyl-4-[3-(tert.butylamino)-2-hydroxypropyl]phenyl]-1,1-diethylurea HCl) (celiprolol);

((±)-2-[2-[3-[(1,1-dimethylethyl)amino]-2-hydroxypropoxy]phenoxy]-N-methylacetamide HCl) (cetamolol);

(2-benzimidazolyl-phenyl(2-isopropylaminopropanol));

((±)-3'-acetyl-4'-(2-hydroxy-3-isopropylaminopropoxy)acetanilide HCl) (diacetolol);

(methyl-4-[2-hydroxy-3-[(1-methylethyl)aminopropoxy]]benzenepropanoate HCl) (esmolol);

(erythro-DL-1-(7-methylindan-4-yloxy)-3-isopropylaminobutan-2-ol);

(1-(tert.butylamino)-3-[O-(2-propynyloxy)phenoxy]-2-propanol (pargolol);

(1-(tert.butylamino)-3-[o-(6-hydrazino-3-pyridazinyl)phenoxy]-2-propanol diHCl) (prizidilol);

((-)-2-hydroxy-5-[(R)-1-hydroxy-2-[(R)-(1-methyl-3-phenylpropyl)amino]ethyl]benzamide);

(4-hydroxy-9-[2-hydroxy-3-(isopropylamino)-propoxy]-7-methyl-5H-furo[3,2-g][1]-benzopyran-5-one) (iprocrolol);

((-)-5-(tert.butylamino)-2-hydroxypropoxy]-3,4-dihydro-1-(2H)-naphthalenone HCl) (levobunolol);

(4-(2-hydroxy-3-isopropylamino-propoxy)-1,2-benzisothiazole HCl);

(4-[3-(tert.butylamino)-2-hydroxypropoxy]-N-methylisocarbostyril HCl);

((±)-N-2-[4-(2-hydroxy-3-isopropyl aminopropoxy)phenyl]ethyl-N'-isopropylurea) (pafenolol);

(3-[[(2-trifluoroacetamido)ethyl]amino]-1-phenoxypropan-2-ol);

(N-(3-(o-chlorophenoxy)-2-hydroxypropyl)-N'-(4'-chloro-2,3-dihydro-3-oxo-5-pyridazinyl)ethylenediamine);

((±)-N-[3-acetyl-4-[2-hydroxy-3-[(1-methylethyl)amino]propoxy]phenyl]butanamide) (acebutolol);

((±)-4'-[3'-(tert-butylamino)-2-hydroxypropoxy]spiro[cyclohexane-1,2'-indan]-1'-one) (spirendolol);

(7-[3-[[2-hydroxy-3-[(2-methylindol-4-yl)oxy]propyl]amino]butyl]thiophylline) (teoprolol);

((±)-1-tert.butylamino-3-(thiochroman-8-yloxy)-2-propanol) (tertatolol);

((±)-1-tert.butylamino-3-(2,3-xylyloxy)-2-propanol HCl) (xibenolol);

(8-[3-(tert.butylamino)-2-hydroxypropoxy]-5-methylcoumarin) (bucumolol);

(2-(3-(tert.butylamino)-2-hydroxy-propoxy)benzonitrile HCl) (bunitrolol);

((±)-2'-[3-(tert-butylamino)-2-hydroxypropoxy-5'-fluorobutyrophenone) (butofilolol);

(1-(carbazol-4-yloxy)-3-(isopropylamino)-2-propanol) (carazolol);

(5-(3-tert.butylamino-2-hydroxy)propoxy-3,4-dihydrocarbostyril HCl) (carteolol);

(1-(tert.butylamino)-3-(2,5-dichlorophenoxy)-2-propanol) (cloranolol);

(1-(inden-4(or 7)-yloxy)-3-(isopropylamino)-2-propanol HCl) (indenolol);

(1-isopropylamino-3-[(2-methylindol-4-yl)oxy]-2-propanol) (mepindolol);

(1-(4-acetoxy-2,3,5-trimethylphenoxy)-3-isopropylaminopropan-2-ol) (metipranolol);

(1-(isopropylamino)-3-(o-methoxyphenoxy)-3-[(1-methylethyl)amino]-2-propanol) (moprolol);

((1-tert.butylamino)-3-[(5,6,7,8-tetrahydro-cis-6,7-dihydroxy-1-naphthyl)oxy]-2-propanol) (nadolol);

((S)-1-(2-cyclopentylphenoxy)-3-[(1,1-dimethylethyl)amino]-2-propanol sulfate (2:1)) (penbutolol);

(4'-[1-hydroxy-2-(amino)ethyl]methanesulfonanilide) (sotalol);

(2-methyl-3-[4-(2-hydroxy-3-tert.butylaminopropoxy)phenyl]-7-methoxy-isoquinolin-1-(2H)-one);

(1-(4-(2-(4-fluorophenyloxy)ethoxy)phenoxy)-3-isopropylamino-2-propanol HCl);

((-)-p-[3-[(3,4-dimethoxyphenethyl)amino]-2-hydroxypropoxy]-β-methylcinnamonitrile) (pacrinolol);

((±)-2-(3'-tert.butylamino-2'-hydroxypropylthio)-4-(5'-carbamoyl-2'-thienyl)thiazole HCl) (arotinolol);

((±)-1-[p-[2-(cyclopropylmethoxy)ethoxy]phenoxy]-3-(isopropylamino)-2-propanol) (cicloprolol);

((±)-1-[(3-chloro-2-methylindol-4-yl)oxy]-3-[(2-phenoxyethyl)amino]-2-propanol) (indopanolol);

((±)-6-[[2-[[3-(p-butoxyphenoxy)-2-hydroxypropyl]amino]ethyl]amino]-1,3-dimethyluracil) (pirepolol);

(4-(cyclohexylamino)-1-(1-naphtholenyloxy)-2-butanol);

(1-phenyl-3-[2-[3-(2-cyanophenoxy)-2-hydroxypropyl]aminoethyl]hydantoin HCl);

(3,4-dihydro-8-(2-hydroxy-3-isopropylaminopropoxy)-3-nitroxy-2H-1-benzopyran) (nipradolol);

α and β-Adrenergic Blocking Agents:

((±)-1-tert-butylamino)-3-[o-[2-(3-methyl-5-isoxazolyl)vinyl]phenoxy]-2-propanol) (isoxaprolol);

(1-isopropylamino-3-(4-(2-nitroxyethoxy)phenoxy)-2-propanol HCl);

4-hydroxy-α-[[3-(4-methoxyphenyl)-1-methylpropyl]aminomethyl]-3-(methylsulfinyl)-benzmethanol HCl) (sulfinalol);

(5-[1-hydroxy-2-[[2-(o-methoxyphenoxy)ethyl]amino]ethyl]-2-methylbenzenesulfonamide HCl);

(5-[1-hydroxy-2-[(1-methyl-3-phenylpropyl)amino]ethyl]salicylamide HCl) (labetalol);

(1-((3-chloro-2-methyl-1H-indol-4-yl)oxy)-3-((2-phenoxyethyl)amino)-2-propanol-hydrogenmalonate) (ifendolol);

(4-(2-hydroxy-3-[(1-methyl-3-phenylpropyl)amino]propoxy)benzeneacetamide);

(1-[3-[[3-(1-naphthoxy)-2-hydroxypropyl]-amino]-3,3-dimethyl-propyl]-2-benzimidazolinone);

(3-(1-(2-hydroxy-2-(4-chlorophenylethyl)-4-piperidyl)-3,4-dihydroxy)quinoxolin-2(1H)-one);

CNS-Acting Agents: clonidine; methyldopa;

Adrenergic Neuron Blocking Agents: guanethidine; reserpine and other rauwolfia alkaloids such as rescinnamine;

Vasodilators: diazoxide; hydralazine; minoxidil;

Angiotensin I Converting Enzyme Inhibitors:

1-(3-mercapto-2-methyl-1-oxopropyl)-L-proline (captopril);

(1-(4-ethoxycarbonyl-2,4(R,R)-dimethylbutanoyl)indoline-2(S)-carboxylic acid);

(2-[2-[[1-(ethoxycarbonyl)-3-phenyl-propyl]amino]-1-oxopropyl]-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid);

((S)-1-[2-[[1-(ethoxycarbonyl)-3-phenylpropyl]amino]-1-oxopropyl]octahydro-1H-indole-2-carboxylic acid HCl);

(N-cyclopentyl-N-(3-(2,2-dimethyl-1-oxopropyl)thiol-2-methyl-1-oxopropyl)glycine) (pivalopril);

((2R,4R)-2-(2-hydroxyphenyl)-3-(3-mercaptopropionyl)-4-thiazolidinecarboxylic acid);

(1-(N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-(S)-alanyl)cis,syn-octahydroindol-2(S)-carboxylic acid HCl);

((-)-(S)-1-[(S)-3-mercapto-2-methyl-1-oxopropyl]indoline-2-carboxylic acid);

([1(S),4S]-1-[3-(benzoylthio)-2-methyl-1-oxopropyl]-4-phenylthio-L-proline;

(3-([1-ethoxycarbonyl-3-phenyl-(1S)-propyl]amino)-2,3,4,5-tetrahydro-2-oxo-1-(3S)-benzazepine-1-acetic acid HCl);

(N-(2-benzyl-3-mercaptopropanoyl)-S-ethyl-L-cysteine) and the S-methyl analogue;

(N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-L-proline maleate) (enalapril);

N-[1-(S)-carboxy-3-phenylpropyl]-L-alanyl-1-proline;

N² -[1-(S)-carboxy-3-phenylpropyl]-L-lysyl-L-proline (lysinopril);

Other Antihypertensive Agents: aminophylline; cryptenamine acetates and tannates; deserpidine; meremethoxylline procaine; pargyline; trimethaphan camsylate;

and the like, as well as admixtures and combinations thereof.

Typically, the individual daily dosages for these combinations can range from about one-fifth of the minimally recommended clinical dosages to the maximum recommended levels for the entities when they are given singly. Coadministration is most readily accomplished by combining the active ingredients into a suitable unit dosage form containing the proper dosages of each. Other methods of coadministration are, of course, possible.

The novel peptides of the present invention possess an excellent degree of activity in treating renin-associated hypertension and hyperaldosteronism.

For these purposes the compounds of the present invention may be administered parenterally, by inhalation spray, or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection or infusion techniques. In addition to the treatment of warm-blooded animals such as mice, rats, horses, dogs, cats, etc., the compounds of the invention are effective in the treatment of humans.

The pharmaceutical compositions may be in the form of a sterile injectable preparation, for example as a sterile injectable aqueous or oleagenous suspension. This suspension may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectibles.

The peptides of this invention may also be administered in the form of suppositories for rectal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Such materials are cocoa butter and polyethylene glycols.

Dosage levels of the order of 0.1 to 4.0 grams per day are useful in the treatment of the above indicated conditions. For example, renin-associated hypertension and hyperaldosteronism are effectively treated by the administration of from 1.0 to 50 milligrams of the compound per kilogram of body weight per day.

The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

Thus, in accordance with the present invention there is further provided a pharmaceutical composition for treating renin-associated hypertension and hyperaldosteronism, comprising a pharmaceutical carrier and a therapeutically effective amount of a peptide of the formula: ##STR29## wherein A, B, D, E, R¹, G, R⁴, J, and L have the same meaning as recited further above for Formula I; wherein all of the asymmetric carbon atoms have an S configuration, except for those in the B, D, and Q portion of G substituents, which may have an S or R configuration; and a pharmaceutically acceptable salt thereof.

Also, in accordance with the present invention there is still further provided a method of treating renin-associated hypertension and hyperaldosteronism, comprising administering to a patient in need of such treatment, a therapeutically effective amount of a peptide of the formula: ##STR30## wherein A, B, D, E, R¹, G, R⁴, J, and L have the same meaning as recited further above for Formula I; wherein all of the asymmetric carbon atoms have an S configuration, except for those in the A, D, and Q portion of G substituents, which may have an S or R configuration; and a pharmaceutically acceptable salt thereof.

The renin inhibitory novel peptides of the present invention may also be utilized in diagnostic methods for the purpose of establishing the significance of renin as a causative or contributory factor in hypertension or hyperaldosteronism in a particular patient. For this purpose the novel peptides of the present invention may be administered in a single dose of from 0.1 to 10 mg per kg of body weight.

Both in vivo and in vitro methods may be employed. In the in vivo method, a novel peptide of the present invention is administered to a patient, preferably by intravenous injection, although parenteral administration is also suitable, at a hypotensive dosage level and as a single dose, and there may result a transitory fall in blood pressure. This fall in blood pressure, if it occurs, indicates supranormal plasma renin levels.

An in vitro method which may be employed involves incubating a body fluid, preferably plasma, with a novel peptide of the present invention and, after deproteinization, measuring the amount of angiotensin II produced in nephrectomized, pentolinium-treated rats. Another in vitro method involves mixing the plasm or other body fluid with a novel peptide of the present invention and injecting the mixture into a test animal. The difference in pressor response with and without added peptide is a measure of the renin content of the plasma.

Pepstatin may be employed in the methods described above as an active control. See, e.g., U.S. Pat. Nos. 3,784,686 and 3,873,681 for a description of the use of pepstatin in diagnostic methods of this type.

The novel peptides of the present invention may be prepared in accordance with well-known procedures for preparing peptides from their constituent amino acids, which will be described in more detail below.

A general method of preparation may be described in the following terms:

A method of preparing a peptide of Formula I, said peptide being comprised of from two to eight amino acids identified as I through VIII, amino acid (AA) I being at the C-terminus of said peptide, to which the substituent L is attached, and amino acid (AA) VIII being at the N-terminus of said peptide, to which substituent A is attached, but also including the peptide isostere component G, comprising the steps of:

(A) treating the desired ester or amide of the C-terminus amino acid (AA I) with the next adjacent amino acid (AA II) of said peptide, or peptide isostere component G, the amino group of said amino acid or isostere being protected by a protecting group, in the presence of a condensing agent, whereby a dipeptide of the two amino acids (AA I and II) or isostere G is formed;

(B) deprotecting the dipeptide formed in Step (A) by removing the protecting group from the amino group of AA II or isostere G;

(C) treating the dipeptide of AA I and AA II or isostere G with AA III, the amino group of which is protected by a protecting group, in the presence of a condensing agent, whereby a tripeptide of AA I, AA II and AA III is formed;

(D) deprotecting the tripeptide formed in Step (C) by removing the protecting group from the amino group of AA III, to give the peptide of Formula I wherein A is hydrogen; the Steps (C) and (D) also optionally being carried out so as to introduce the peptide isostere component G;

(E) treating the tripeptide formed in Step (D) where an ester of AAI is employed with hydrazine to give the corresponding hydrazide, followed by treatment of said hydrazine with acidic nitrite to give the corresponding acyl azide, followed by treatment of said acyl azide with the appropriate amine compound to give the desired J substituent which is not an amino acid, in the peptide of Formula I; and optionally

(F) treating the tripeptide formed in Step (E) with ##STR31## where X, R_(a) ², and R_(b) ², are as defined above and W is an acid halide, anhydride, or other carbonyl activating group, to give the peptide of Formula I wherein A is other than hydrogen; and optionally

(G) forming a tetrapeptide up to an octapeptide of AA I, through AA VIII, by repeating the procedure of Step (C) using protected AA IV through protected AA VIII;

(H) deprotecting the tetrapeptide through octapeptide formed in Step (G) to give the peptide of Formula I wherein A is hydrogen; and optionally

(I) treating the tetrapeptide through octapeptide from in Step (H) with ##STR32## where X, R_(a) ², and R_(b) ² are as defined above and W is an acid halide, anhydride, or other carboxyl activating group, to give the peptide of Formula I wherein A is other than hydrogen;

said method also comprising, where necessary, protection of sidechain substituents of the component amino acids AA I through AA V, with deprotection being carried out as a final step; said method also comprising any combination of the steps set out above, whereby the amino acids I through VIII and substituents A, B, D, E, G, J, and L are assembled in any desired order to prepare the peptide of Formula I; and said method also comprising employment of the steps set out above in a solid phase sequential synthesis, whereby in the initial step the carboxyl group of the selected amino acid is bound to a synthetic resin substrate while the amino group of said amino acid is protected, followed by removal of the protecting group, the succeeding steps being as set out above, the peptide as it is assembled being attached to said synthetic resin substrate; followed by a step of removing the peptide of Formula I from said synthetic resin substrate: (a) by strong acid cleavage to give L═OH; (b) by transesterification with a C₁₋₄ alkanol to give L═O--C₁₋₄ alkyl (followed by hydrolysis to give L═OH); or (c) by ammonolysis with NH₂ R" where R" is hydrogen or C₁₋₄ alkyl; said removal steps also optionally being carried out as treatments where solid phase sequential synthesis is not carried out, to give the same L substituent endings; and after removal of the peptide of Formula I from said synthetic resin substrate by transesterification to form the ester thereof as recited above, optionally the step of teating said ester thereof in accordance with the procedures described in Step (E) above to give the desired J substituent in the peptide of Formula I; removal of sidechain protecting groups being accomplished either before or after removal of the peptide of Formula I from said synthetic resin substrate.

Preparation of the peptides of Formula I having the desired J substituent, as described above in Step (E), may be illustrated as follows for the particular case where J=benzylamide (and PEP represents the remaining portion of the peptide of Formula I): ##STR33##

The isostere component G of the novel peptides of the present invention may be prepared in accordance with well-known procedures in synthetic chemistry, as is described in more detail further below. Attachment of the isostere component G to the other components of the novel peptides of the present invention is carried out in the same manner as for any of said other components, and may involve addition of the isostere component in a protected form. For example, the following reactive groups would require such protection: ##STR34## Such protecting groups may be removed as a final or near final step, for example, by base hydrolysis in the former case, or by hydrogenation in the latter.

Preparation of the particular isostere components may be carried out in accordance with procedures described below and in the literature cited particularly as follows: ##STR35## Ondetti et al., Chemistry and Biology of Peptides, ed. J. Meienhofer, Ann. Arbor Science pp. 525-531, 1972. ##STR36##

(1) Natarajan et al., Peptides. Synthesis-Structure-Function, ed. D. H. Rich and E. Gross, Pierce Chem. Co., Rockford, Ill., pp. 429-433, 1981.

(2) Van Lommen et al., European Peptide Symposium 16th, Peptides 1980, ed. K. Brunfeldt, Scriptor, Copenhagen, pp. 248-252, 1981.

(3) Almquist et al., J. Med. Chem., 23: 1392-1398, 1980. ##STR37## Kawasaki and Maeda, Biochem. Biophys. Res. Comm. 106: 113-116, 1982. ##STR38##

(1) Natarajan et al., Id.

(2) Fok and Yankellov, Biochem. Biophys. Res. Comm. 74: 273-278, 1977.

(3) Spatola et al., Peptides. Structure-Function-Biological Function, ed. E. Gross and J. Meienhofer, Pierce Chem. Co., Rockford, 111., pp. 273-276, 1979.

(4) Spatola and Bettag, J. Org. Chem. 46: 2393-2394, 1981. ##STR39##

(1) Natarajan et al., Id.

(2) Hann et al., J. Chem. Soc. Chem. Comm., 234-235, 1980.

(3) Cox et al., J. Chem. Soc. Chem. Comm., 799-800, 1980. ##STR40## Ondetti et al., Id. ##STR41##

(1) Van Lommen et al., Id.

(2) Atherton et al., J. Chem. Soc. (C), 3393-3396, 1971.

(3) Parry et al., Chemistry and Biology of Peptides, ed. J. Meienhofer, Ann Arbor Science, pp. 541-544, 1972.

(4) Hudson et al., Int. J. Peptide Protein Res. 15: 122-129, 1979.

(5) Frank and Desiderio, Anal. Biochem. 90: 413-419, 1978. ##STR42##

Prepared from ketomethylene by Wittig reaction. ##STR43##

(1) Jacobson and Bartlett, JACS 103: 654-657, 1981.

(2) Jennings-White and Almquist, Tet. Lett., 23: 2533-2534, 1982.

(3) Morton et al., Tet. Lett., 23: 4123-4126, 1982.

For example, the compound: ##STR44## can be prepared in accordance with the following scheme: ##STR45## which can be incorporated into the synthesis for the overall peptide of the present invention, or converted to the α-BOC derivative by hydrogenation over Pd/C catalyst, followed by treatment with (BOC)₂ O. Incorporation of (III.) or its BOC analog into a peptide sequence gives, after alkaline hydrolysis of the phosphinate ester, the free phosphinate ##STR46## The product will contain two isomers at *; the active diastereomer has the relative configuration as an L amino acid, i.e., R-isomer in this case. Also, the compound: ##STR47## can be prepared in a fashion analgous to that described for (III.) above, for example from: ##STR48## Incorporation of (IV.) or its N-BOC analog proceeds as for (III.) above, with removal of the methyl phosphinate ester by hydrolysis (alkaline) to give the free phosphinate. The active isomer shown at * has the side chains in the relative configuration of the dipeptide that they mimic. For synthesis of ##STR49## See Jennings-White and Almquist, Id. ##STR50##

Morton et al., Id.

For example, the compound: ##STR51## can be prepared in accordance with the following scheme: ##STR52## The sulfone ##STR53## can be obtained using excess m-Cl-perbenzoic acid.

Use of ##STR54## in the second step gives as the final product: ##STR55##

Prepared from the alcohol; see Rich et al., Biochem. Biophys. Res. Comm. 104: 1127-1133, 1982.

Conversion of the ketone to the thioketone is with use of: ##STR56##

Obtained from ##STR57## in accordance with the scheme outlined below; the R substituents are attached by conventional methods to the free amine (R═H):

Synthesis of protected 3-amino-3-deoxy-(3S,4S)Statine: ##STR58## Base hydrolysis gives the free acid for incorporation into the synthesis of the overall peptides of the present invention. ##STR59##

Obtained from the amide according to the method described by Clausen et al., Seventh American Peptide Symposium, 1981: ##STR60## (1) Natarajan et al., Id. (2) Fok and Yankellov, Id.

(3) Spatola et al., Id.

(4) Spatola and Bettag, Id.

(5) Spatola et al. Proceedings of the Seventh American Peptide Symposium, ed. E. Gross and D. H. Rich. pp. 613-616, 1981.

The novel inhibitory peptides of the present invention are prepared by using the solid phase sequential synthesis technique.

In the following description several abbreviated designations are used for the amino acid components, certain preferred protecting groups, reagents and solvents. The meanings of such abbreviated designations are given below in Table I.

                  TABLE I                                                          ______________________________________                                         Abbreviated                                                                    Designation                                                                    ______________________________________                                                          Amino Acid                                                    Ala              L-alanine                                                     Arg              L-arginine                                                    Gly              L-glycine                                                     His              D or L-histidine                                              Ile              L-isoleucine                                                  Leu              L-leucine                                                     Lys              L-lysine                                                      Met              L-methionine                                                  Orn              L-ornithine                                                   Phe              L-phenylalanine                                               Ser              L-serine                                                      Sar              L-sarcosine                                                   (N--methylglycine)                                                             Thr              L-threonine                                                   Trp              L-tryptophan                                                  Tyr              L-tyrosine                                                    Val              L-valine                                                                       Protecting                                                                     Groups                                                        BOC              tert-butyloxycarbonyl                                         CBZ              benzyloxycarbonyl                                             DNP              dinitrophenyl                                                 OMe              methyl ester                                                                   Activating                                                                     Groups                                                        HBT              1-hydroxybenzotriazole                                                         Condensing                                                                     Agents                                                        DCCI             dicyclohexylcarbodiimide                                      DPPA             diphenylphosphorylazide                                                        Reagents                                                      TEA              triethylamine                                                 TFA              trifluoroacetic acid                                                           Solvents                                                      A                ammonium hydroxide (conc.)                                    AcOH             acetic acid                                                   C                chloroform                                                    DMF              dimethylformamide                                             E                ethyl acetate                                                 M                methanol                                                      P                pyridine                                                      THF              tetrahydrofuran                                               W                water                                                         ______________________________________                                    

The synthesis of the peptides of the present invention by the solid phase technique is conducted in a stepwise manner on chloromethylated resin. The resin is composed of fine beads (20-70 microns in diameter) of a synthetic resin prepared by copolymerization of styrene with 1-2 percent divinylbenzene. The benzene rings in the resin are chloromethylated in a Friedel-Crafts reaction with chloromethyl methyl ether and stannic chloride. The Friedel-Crafts reaction is continued until the resin contains 0.5 to 5 mmoles of chlorine per gram of resin.

The amino acid selected to be the C-terminal amino acid of the linear peptide is converted to its amino protected derivative. The carboxyl group of the selected C-terminal amino acid is bound covalently to the insoluble polymeric resin support, as for example, as the carboxylic ester of the resin-bonded benzyl chloride present in chloromethyl-substituted polystyrene-divinylbenzene resin. After the amino protecting group is removed, the amino protected derivative of the next amino acid in the sequence is added along with a coupling agent, such as dicyclohexylcarbodiimide. The amino acid reactant may be employed in the form of a carboxyl-activated amino acid such as ONP ester, an amino acid azide, and the like. Deprotection and addition of successive amino acids is performed until the desired linear peptide is formed.

The selection of protecting groups is, in part, dictated by particular coupling conditions, in part by the amino acid and peptide components involved in the reaction.

Amino-protecting groups ordinarily employed include those which are well known in the art, for example, urethane protecting substituents such as benzyloxy-carbonyl (carbobenzoxy), p-methoxycarbobenzoxy, p-nitrocarbobenzoxy, t-butyoxycarbonyl, and the like. It is preferred to utilize t-butyloxycarbonyl (BOC) for protecting the α-amino group in the amino acids undergoing reaction at the carboxyl end of said amino acid. The BOC protecting group is readily removed following such coupling reaction and prior to the subsequent step by the relatively mild action of acids (i.e. trifluoroacetic acid, or hydrogen chloride in ethyl acetate).

The OH group of Thr and Ser can be protected by the Bzl group and the -amino group of Lys can be protected by the INOC group or the 2-chlorobenzyloxycarbonyl (2-Cl-CBZ) group. Neither group is affected by TFA, used for removing BOC protecting groups. After the peptide is formed, the protective groups, such as 2-Cl-CBZ and Bzl, can be removed by treatment with HF or by catalytic hydrogenation.

After the peptide has been formed on the solid phase resin, it may be removed from the resin by a variety of methods which are well known in the art. For example the peptide may be cleaved from the resin with hydrazine, by ammonia in methanol, or by methanol plus a suitable base.

Preparation of the novel inhibitory peptides of the present invention utilizing the solid phase technique is illustrated in the following examples, which however, are not intended to be any limitation of the present invention.

EXAMPLE 1 tert-Butyloxycarbonyl-L-histidyl-L-prolyl-L-homophenylalanyl-L-histidyl-(2S,4S,5S)-5-amino-6-cyclohexyl-4-hydroxyl-2-isopropylhexanoyl-L-isoleucyl-(4-pyridyl)-methylamide ##STR61##

The title peptide is prepared by a combination of solid phase and solution methods.

Step A: BOC-L-His-L-Pro-L-Homophe-L-His-(2S,4S,5S)-5-amino-6-cyclohexyl-(O-benzyl)-4-hydroxyl-2-isopropylhexanoyl-L-Ile-O-Resin

The title peptide resin is prepared by standard solid phase methodology, as described in Erickson and Merrifield, Proteins, 3rd, Ed., 2 257-527, 1971, using a Beckman 990B peptide synthesizer to carry out the operations according to the attached programs. The starting polymer is BOC-L-Ile esterified to 2% cross-linked polystyrenedivinylbenzene (2 mmole, 2.5 g). The N-BOC derivatives of His (DNP). Pro and L-HPhe are coupled using DCC with an equivalent amount of the additive 1-hydroxybenzotriazole. The N-BOC, O-benzyl derivative of the dipeptide isostere, (2S,4S,5S)-5-amino-6-cyclohexyl-4-hydroxyl-2-isopropylhexanoic acid (ACHiPHA) is prepared as described separately according to a variation of the scheme described by Szelke et al., (M. Szelde, D. M. Jones, B. Abash, A. Hallett, and B. J. Leckie, in Proceedings of the Eighth American Peptide Symposium, in press, presented 26 May 1983 at Tucson, Ariz.) This derivative is coupled as done for the α-amino acid derivatives, using DCC and HBT, except only 1.25 excess is used, coupling for 18 hours, followed by a recoupling, using the saved coupling solution, for an additional 18 hours. This is done to preserve the supply of this derivative. During the growth of the peptide resin, BOC-removal is accomplished with 40% trifluoroacetic acid in CH₂ Cl₂. For the coupling of amino acids, a coupling of 30 minutes followed by a recoupling of 60 minutes (2.5 equivalents each time of BOC-amino acid, in 1:1 CH₂ Cl₂ :DMF), is used, except as noted above. The DNP protecting groups on His are removed at the final step in the synthesis using 10% thiophenol in DMF according to the following program.

    ______________________________________                                         SCHEDULE OF STEPS FOR 2 MMOL RUN                                               Step Solvent/Reagent  Vol. (ml)  Mix time (min)                                ______________________________________                                         Coupling Program 1                                                             1    CH.sub.2 Cl.sub.2                                                                               6 × 20                                                                              2                                             2    40% TFA in CH.sub.2 Cl.sub.2                                                                    1 × 20                                                                              2                                             3    40% TFA in CH.sub.2 Cl.sub.2                                                                    1 × 20                                                                              25                                            4    CH.sub.2 Cl.sub.2                                                                               3 × 20                                                                              2                                             5    10% TEA in CH.sub.2 Cl.sub.2                                                                    2 × 20                                                                              5                                             6    CH.sub.2 Cl.sub.2                                                                               3 × 20                                                                              2                                             7    BOC--amino acid, HBT                                                                            20         5                                                  in 1:1 DMF/CH.sub.2 Cl.sub.2                                              8    1.0 M DCCI in CH.sub.2 Cl.sub.2                                                                  5         60                                            9    DMF              1 × 20                                                                              2                                             10   MeOH             2 × 20                                                                              2                                             11   CH.sub.2 Cl.sub.2                                                                               1 × 20                                                                              2                                             Re-Couple Program 2                                                            1    CH.sub.2 Cl.sub.2                                                                               1 × 20                                                                              2                                             2    10% TEA in CH.sub.2 Cl.sub.2                                                                    2 × 20                                                                              5                                             3    CH.sub.2 Cl.sub.2                                                                               3 × 20                                                                              2                                             4    BOC--amino acid, HBT                                                                            40         5                                                  in 1:1 DMF/CH.sub.2 Cl.sub.2                                              5    1.0 M DCCI in CH.sub.2 Cl.sub.2                                                                  5         120                                           6    DMF              1 × 20                                                                              2                                             7    MeOH             2 × 20                                                                              2                                             8    CH.sub.2 Cl.sub.2                                                                               5 × 20                                                                              2                                             Program 3 (DNP removal)                                                        1    CH.sub.2 Cl.sub.2                                                                               1 × 20                                                                              2                                             2    DMF              2 × 20                                                                              2                                             3    10% phenylthiol in DMF                                                                          1 × 20                                                                              25                                            4    DMF              1 × 20                                                                              2                                             5    10% TEA in CH.sub.2 Cl.sub.2                                                                    1 × 20                                                                              2                                             6    DMF              2 × 20                                                                              2                                             7    10% phenylthiol in DMF                                                                          1 × 20                                                                              25                                            8    DMF              3 × 20                                                                              2                                             9    MeOH             2 × 20                                                                              2                                             10   CH.sub.2 Cl.sub.2                                                                               2 × 20                                                                              2                                             11   MeOH             2 × 20                                                                              2                                             12   CH.sub.2 Cl.sub.2                                                                               2 × 20                                                                              2                                             13   MeOH             2 × 20                                                                              2                                             ______________________________________                                    

Step B: BOC-L-His-L-Pro-L-HPhe-L-His-(2S,4S,5S)-ACHiPHA-Ile-hydrazide

The peptide resin A is stirred in 50 ml dry methanol plus 10 ml diisopropylethylamine for 18 hours. The suspension is filtered and the filtrate evaporated. The resin is resuspended as before and stirred for another 18 hours. After filtration, the combined filtrates are evaporated, dissolved in ethyl acetate and washed with water repeatedly. The ethyl acetate layer is dried over Na₂ SO₄ and evaporated. The residue is dissolved in 20 ml MeOH and hydrogenated for 5 hours over 40 lbs. H₂ and 0.1 g Pd/C catalyst. This removes the O-benzyl protecting group on ACHiPHA. The product, BOC-His-Pro-HPhe-His-ACHiPHA-Ile-OCH₃, is dissolved in 20 ml 1:1 hydrazine:methanol for 30 minutes. The solvent is removed under pressure at 40° C. and the residue dissolved in EtAc and washed repeatedly with water to remove traces of hydrazine. The EtAc layer is then dried over Na₂ SO₄ and evaporated to give the title material as a powder.

Step C ##STR62##

An approximately 0.5 mmole portion of the crude B is dissolved in 20 ml DMF and cooled under N₂ to -30° C. To this solution is added 5 mmole (10 equivalents) of HCl in dioxane solution. Then, to this mixture is added 0.5 mmole isoamylnitrite. Additionally, 0.05 mmole portions are added if necessary to maintain a slight excess of nitrite as determined by 10N HCl-moistened starch-KI paper. After 30 minutes, 5 mmole of diisopropylethylamine is added, followed by 2 equivalents (1.0 mmole) of 4-aminomethylpyridine. The pH, as judged on water-moistened pH paper, is maintained at 7 and 9 by additions of DIPEA for the next 6 hours. Evaporation of the solution and washing in EtAc (containing 10% MeOH) with water, gives, after drying and evaporation of the organic layer, the crude title peptide derivative. This crude material is readily purified using silica gel chromatography to give the title material.

EXAMPLE 2 N.sup.α -tert-Butyloxycarbonyl-(2S, 4S,5S)-5-amino-6-cyclohexyl-(O-benzyl)-4-hydroxyl-2-isopropyl-hexanoic acid

The title dipeptide analog is prepared according to the scheme outlined by Szelke (see reference in Example 1) for a similar compound. The starting material, L-cyclohexylalanine, is readily prepared from L-phenylalanine by reduction using Rh/Alumina and H₂ (40 lb) in EtOH/H₂ O. ##STR63##

For other hydroxyethylene analogs, the configuration preferred is the same relative one as shown here, but by R-S nomenclature may be different at the 2-center.

Thus, preferred is ##STR64## which is 2-S but ##STR65## which is the same relative conuration but is formally 2-R. Abbreviations:

DIPEA=diisopropylethylamine

Bzl=benzyl

Et=ethyl

tBu=tert-butyl ##STR66## =cyclohexyl

EXAMPLES 3-17

Following the standard solid phase methodology described above in Example 1, additional inhibitory peptides of the present invention can be prepared. Such peptides prepared are set out in the following table.

    __________________________________________________________________________     Ex. No.                                                                             Peptide                                                                   __________________________________________________________________________      3.                                                                                  ##STR67##                                                                 4.                                                                                  ##STR68##                                                                 5.                                                                                  ##STR69##                                                                 6.                                                                                  ##STR70##                                                                 7.                                                                                  ##STR71##                                                                 8.                                                                                  ##STR72##                                                                 9.                                                                                  ##STR73##                                                                10.                                                                                  ##STR74##                                                                      ##STR75##                                                                      ##STR76##                                                                      ##STR77##                                                                      ##STR78##                                                                      ##STR79##                                                                      ##STR80##                                                                      ##STR81##                                                                __________________________________________________________________________ 

What is claimed is:
 1. A peptide of the formula: ##STR82## wherein: A is hydrogen; or ##STR83## where X is ##STR84## and R_(a) ² and R_(b) ² may be the same or different and are hydrogen; W--(CH₂)_(n) -- or W--(CH₂)_(m) --CH═CH--(CH₂)_(p) --, where W is hydrogen; C₁₋₄ alkyl; aryl; C₃₋₇ cycloalkyl; or C₃₋₇ cycloalkyl or aryl substituted with up to five members independently selected from the group consisting of C₁₋₈ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; n is 0 to 5; m is 0 to 2; and p is 0 to 2; except that where X is --O--, only one of R_(a) ² or R_(b) ² is present;B is absent; glycyl; sarcosyl; or ##STR85## where R¹ is as defined further below; D is absent; or ##STR86## where Z is --(CH₂)₁ -- and 1 is 1 or 2; or --S--; E is absent; or ##STR87## where m is 1 to 4; and R⁵ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl where the aryl portion is substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl; G is ##STR88## where q is 1 to 4; R⁴ is as defined further below; R⁶ is C₃₋₆ alkyl; C₃₋₇ cycloalkyl; aryl; or C₃₋₇ cycloalkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; and Q is ##STR89## wherein X' is hydroxy; amino; or monoor di-C₁₋₄ alkyl amino; and W' is absent; --O--; --NH--; or --CH₂ --; ##STR90## where X" and X"' are independently absent; or ##STR91## and W" is absent; --CH₂ --; or ##STR92## where R⁸ is hydrogen or C₁₋₃ alkyl; ##STR93## where R is hydrogen; C₁₋₄ alkyl; formyl; C₁₋₄ alkanoyl; aroyl; carboxy; C₁₋₄ alkoxycarbonyl; aryloxycarbonyl; or aryl C₁₋₄ alkoxycarbonyl; J is (1) --Y--(CH₂)_(n) --R⁷ where Y is --NH-- or --O--; n is 0 to 5; and R⁷ is hydrogen; hydroxy; C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- or di-C₁₋₄ alkylamino, and halo; amino; mono-, di-, or tri-C₁₋₄ alkylamino; guanidyl; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino; (2) ##STR94## where Y is as defined above; n is 0 or 1; n' is 0 or 1; n" is 1 to 4; n'" is 1 to 4; and R⁷ and R_(a) ⁷ may be the same or different and have the same meaning as R⁷ above and R_(a) ⁷ may additionally be ##STR95## where R⁸ is hydrogen or C₁₋₃ alkyl; (3) ##STR96## where Y is as defined above; n is 0 or 1; and Z' is ##STR97## where n is 0 or 1; and R⁸ is as defined above; or ##STR98## where n is 0 or 1; (4) ##STR99## where R⁵ is as defined above; and R⁹ is hydrogen; C₁₋₄ alkyl; hydroxy; or C₃₋₇ cycloalkyl; or (5) ##STR100## where Y is --NH-- or --O--; q is 1-5; q' is 0-5; R¹⁰ is hydrogen; hydroxy; N(R')₂, where R' may be the same or different and is hydrogen or C₁₋₄ alkyl; quanidyl; or N.sup.⊕ (R')₃ A.sup.⊖, where R' is as defined above, and A.sup.⊖ is a counterion; provided that at least one R¹⁰ is not hydrogen; R¹¹ is C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to three members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- or di-C₁₋₄ alkylamino, amino C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ -alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; carboxy, ester or amide; sulfo; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino; R¹² is hydrogen; or carboxy, ester or amide; R¹³ is carboxy, ester or amide; sulfo; or aryl substituted with up to three members selected from the group consisting of amino-C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ -alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; and R¹⁴ is carboxy, ester or amide; or ##STR101## where Y is --NH-- or --O--; k is 0-4; k' is 0 or 1; k" is 0-4; k"' is 0 or 1; R' is hydrogen or C₁₋₄ alkyl; and R" is hydrogen or C₁₋₄ alkyl; L is absent; OR"; NHR"; or N(R")₂, where R" may be the same or different and is hydrogen or C₁₋₄ alkyl; provided that, B and/or L are/is present only when J is defined as in (4) thereunder; R¹ is hydrogen; C₁₋₄ alkyl; hydroxy C₁₋₄ alkyl; aryl; aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; indolyl; 4-imidazolyl; amino C₂₋₄ alkyl; acyl C₂₋₄ alkyl wherein the acyl is ##STR102## and R⁹ is as defined above; guanidyl C₂₋₃ alkyl; or methylthiomethyl; R⁴ is hydrogen; or ##STR103## where R⁹ is as defined above; and R³ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl; andwherein all of the asymmetric carbon atoms have an S configuration, except for those in the B, D, and Q portion of G substituents, which may have an S or R configuration; and a pharmaceutically acceptable salt thereof.
 2. A peptide according to claim 1 wherein the peptide is a member selected from the group consisting essentially of: ##STR104##
 3. A pharmaceutical composition for treating renin-associated hypertension or renin-associated hyperaldosteronism, comprising a pharmaceutical carrier and a therapeutically effective amount of a peptide of the formula: ##STR105## wherein: A is hydrogen; or ##STR106## wherein X is ##STR107## and R_(a) ² and R_(b) ² may be the same or different and are hydrogen; W--(CH₂)_(n) -- or W--(CH₂)_(m) --CH═CH--(CH₂)_(p), where W is hydrogen; C₁₋₄ alkyl; aryl; C₃₋₇ cycloalkyl; or C₃₋₇ cycloalkyl, or aryl substituted with up to five members independently selected from the group consisting of C₁₋₈ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; n is 0 to 5; m is 0 to 2; and p is 0 to 2; except that where X is --O--, only one of R_(a) ² or R_(b) ² is present;B is absent; glycyl; sarcosyl; or ##STR108## where R¹ is as defined further below; D is absent; or ##STR109## where Z is --(CH₂)₁ -- and 1 is 1 or 2; or --S--; E is absent; or ##STR110## where m is 1 to 4; and R⁵ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl where the aryl portion is substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl; G is ##STR111## where q is 1 to 4; R⁴ is as defined further below; R⁶ is C₃₋₆ alkyl; C₃₋₇ cycloalkyl; aryl; or C₃₋₇ cycloalkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; and Q is ##STR112## wherein X' is hydroxy; amino; or mono- or di-C₁₋₄ alkyl amino; and W' is absent; --O--; --NH--; or --CH₂ --; ##STR113## where X" and X"' are independently absent; or ##STR114## and W" is absent; --CH₂ --; or ##STR115## where R⁸ is hydrogen or C₁₋₃ alkyl; ##STR116## where R is hydrogen; C₁₋₄ alkyl; formyl; C₁₋₄ alkanoyl; aroyl; carboxy; C₁₋₄ alkoxycarbonyl; aryloxycarbonyl; or aryl C₁₋₄ alkoxycarbonyl; J is (1) --Y--(CH₂)_(n) --R⁷ where Y is --NH-- or --O--; n is 0 to 5; and R⁷ is hydrogen; hydroxy; C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- or di-C₁₋₄ alkylamino, and halo; amino; mono-, di-, or tri-C₁₋₄ alkylamino; guanidyl; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino; (2) ##STR117## where Y is as defined above; n is 0 or 1; n' or 0 or 1; n" is 1 to 4; n'" is 1 to 4; and R⁷ and R_(a) ⁷ may be the same or different and have the same meaning as R⁷ above and R_(a) ⁷ may additionally be ##STR118## where R⁸ is hydrogen or C₁₋₃ alkyl; (3) ##STR119## where Y is as defined above; n is 0 or 1; and Z' is ##STR120## where n is 0 or 1; and R⁸ is as defined above; or ##STR121## where n is 0 or 1; (4) ##STR122## where R⁵ is as defined above; and R⁹ is hydrogen; C₁₋₄ alkyl; hydroxy; or C₃₋₇ cycloalkyl; or (5) ##STR123## where Y is --NH-- or --O--; q is 1-5; q' is 0-5; R¹⁰ is hydrogen; hydroxy; N(R')₂, where R' may be the same or different and is hydrogen or C₁₋₄ alkyl; guanidyl; or N.sup.⊕ (R')₃ A.sup.⊖, where R' is as defined above, and A.sup.⊖ is a counterion; provided that at least one R¹⁰ is not hydrogen; R¹¹ is C₁₋₄ alkyl; C₃₋₇ cycloalkyl; aryl; aryl substituted with up to three members independently selected from the group consisting of C₁₋₆ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, amino, mono- or di-C₁₋₄ alkylamino, amino C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; carboxy, ester or amide; sulfo; heterocyclic; or heterocyclic substituted with up to five members independently selected from the group consisting of C₁₋₆ alkyl, hydroxy, trifluoromethyl, C₁₋₄ alkoxy, halo, aryl, aryl C₁₋₄ alkyl, amino, and mono- or di-C₁₋₄ alkylamino; R¹² is hydrogen; or carboxy, ester or amide; R¹³ is carboxy, ester or amide; sulfo; or aryl substituted with up to three members selected from the group consisting of amino-C₁₋₄ alkyl, mono-, di-, or tri-C₁₋₄ -alkylamino-C₁₋₄ alkyl, halo, carboxy, carboxy ester or amide, carboxy-C₁₋₄ alkoxy, carboxy-C₁₋₄ alkoxy ester or amide, α-aminocarboxy-C₁₋₄ alkyl, α-aminocarboxy-C₁₋₄ alkyl ester or amide, carboxy-C₁₋₄ alkyl, carboxy-C₁₋₄ alkyl ester or amide, guanidyl, and guanidyl-C₁₋₄ alkyl; and R¹⁴ is carboxy, ester or amide; or ##STR124## where Y is --NH-- or --O--; k is 0-4; k' is 0 or 1; k" is 0-4; k"' is 0 or 1; R' is hydrogen or C₁₋₄ alkyl; and R" is hydrogen or C₁₋₄ alkyl; L is absent; OR"; NHR"; or N(R")₂, where R" may be the same or different and is hydrogen or C₁₋₄ alkyl; provided that, B and/or L are/is present only when J is defined as in (4) thereunder; R¹ is hydrogen; C₁₋₄ alkyl; hydroxy C₁₋₄ alkyl; aryl; aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; indolyl; 4-imidazolyl; amino C₂₋₄ alkyl; acyl C₂₋₄ alkyl wherein the acyl is ##STR125## and R⁹ is as defined above; guanidyl C₂₋₃ alkyl; or methylthiomethyl; R⁴ is hydrogen; or ##STR126## where R⁹ is as defined above; and R³ is hydrogen; C₁₋₄ alkyl; aryl; aryl C₁₋₄ alkyl; aryl C₁₋₄ alkyl or aryl substituted with up to three members selected from the group consisting of C₁₋₄ alkyl, trifluoromethyl, hydroxy, C₁₋₄ alkoxy, and halo; or indolyl; andwherein all of the asymmetric carbon atoms have an S configuration, except for those in the B, D, and Q portion of G substituents, which may have an S or R configuration; and a pharmaceutically acceptable salt thereof.
 4. A composition according to claim 3 wherein the peptide is a member selected from the group consisting essentially of: ##STR127## 